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  1. MCE Kits
  2. Ultra High Sensitivity ECL Kit

Ultra High Sensitivity ECL Kit 

Cat. No.: HY-K1005
ManualSDS

MCE Ultra High Sensitivity ECL Kit is an ultra-sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) labeled antibodies on western blots.

Ultra High Sensitivity ECL Kit

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

SizePriceStock
100  mL ¥650Get quote
500  mL ¥2900Get quote

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

MCE Ultra High Sensitivity ECL Kit enables detection at femtogram range of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems. MCE Ultra High Sensitivity ECL Kit produces a strong, long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins.

Storage

Stored at 4°C, and is stable for 1 year.

Protect from light.

Protocol

1   Take MCE Ultra High Sensitivity ECL Kit out of the refrigerator and allow it to equilibrate at room temperature for about 20 minutes.

2   Prepare the ECL working solution.

Note: Approximately 0.1 mL of ECL Reagent is required per cm² of membrane area.

a)   Mix solutions A and B in a 1:1 ratio. Use a in sufficient volume to ensure that the blot is completely wetted with the ECL working solution.

b)   It is recommended to prepare the working solution just before use, although the mixed reagent solution is stable for several hours at room temperature.

3   Place the blot, protein-side up, in a clean container, and add ECL working solution.

4   Incubate the blot for 1-2 minutes at room temperature.

5   Drain the excess substrate and place the blot in a plastic membrane protector. Remove all air bubbles between the blot and the surface of the membrane protector.

6   Visualize the blot using X-ray film or a CCD-based imaging system.

Components
ComponentsHY-K1005-100 mLHY-K1005-500 mL
Solution A50 mL50 mL × 5
Solution B50 mL50 mL ×5
Documentation

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Inquiry Information

产品名称:
Ultra High Sensitivity ECL Kit
目录号:
HY-K1005
需求量: